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| Registros recuperados : 27 | |
| 1. |  | TAKANOHASHI, C. E.; GODOY, C. V.; KLINGELFUSS, L. H.; CANTERI, M. G. Avaliação da eficiência de fungicidas no controle da ferrugem asiática (Phakopsora pachyrhizi). Fitopatologia Brasileira, Brasília, v. 29, p. S241-S242, ago. 2004. Suplemento. Resumo apresentado no XXXVII Congresso Brasileiro de Fitopatologia, Gramado, RS, agosto, 2004.| Biblioteca(s): Embrapa Soja. |
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| 2. | | YORINORI, J, T.; KIIHL, R. A. S.; ALMEIDA, L. A. de; HIROMOT, D. M.; ROIM, F. L. B.; KLINGELFUSS, L. H. Avaliação da reação de genótipos de soja às doenças fúngicas e variabilidade patogênica (04.1999.335-03). In: EMBRAPA SOJA. Resultados de pesquisa da Embrapa Soja, 1999. Londrina, 2000. p. 59-64. (Embrapa Soja. Documentos, 142).| Biblioteca(s): Embrapa Soja. |
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| 5. | | YORINORI, M. A.; KLINGELFUSS, L. H.; YORINORI, J. T. Efeito da época de aplicação de fungicida e de nutriente foliar sobre o oídio da soja. Fitopatologia Brasileira, Brasília, v. 27, p. S179, ago. 2002. Suplemento. Resumo 570. Edição do XXXV Congresso Brasileiro de Fitopatologia, Recife, PE, ago. 2002.| Biblioteca(s): Embrapa Soja. |
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| 10. | | CAUS, J.; GODOY, C. V.; KLINGELFUSS, L. H.; CANTERI, M. G. Escala diagramática e imagens digitais para avaliar a desfolha em tratamento para controle da ferrugem da soja. Fitopatologia Brasileira, Brasília, v. 29, p. S236, ago. 2004. Suplemento. Resumo apresentado no XXXVII Congresso Brasileiro de Fitopatologia, Gramado, RS, agosto, 2004.| Biblioteca(s): Embrapa Soja. |
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| 11. | | KLINGELFUSS, L. H.; YORINORI, J. T.; DESTRO, D. Identification methods for resistance in soybean to Fusarium solani f. sp. glycines, under greenhouse conditions. In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. p. 79. (Embrapa Soja. Documentos, 228). Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.| Biblioteca(s): Embrapa Soja. |
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| 12. | | LIMA, M. L. R. Z. da C.; MAY, L. L.; SCHUTA, L. R.; KLINGELFUSS, L. H.; LIMA NETO, V. da C. Incidncia de doencça em plantas ornamentais na região metropolitana de Curitiba. Revista do Setor de Ciências Agrárias, Curitiba, v. 15, n. 1, p. 151-154, 1996.| Biblioteca(s): Embrapa Florestas. |
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| 19. | | KLINGELFUSS, L. H.; YORINORI, J. T.; DESTRO, D. Métodos de inoculação para quantificação de resistência em soja a Fusarium solani f. sp. glycines, em casa-de-vegetação. Fitopatologia Brasileira, Brasília, DF, v. 32, n.1, p. 50-55, jan./fev. 2007.| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 27 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
05/03/2012 |
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Data da última atualização: |
19/01/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
WIEBKE-STROHM, B.; PASQUALI, G.; MARGIS-PINHEIRO, M.; BENCKE, M.; BÜCKER-NETO, L.; BECKER-RITT, A. B.; MARTINELLI, A. H. S.; RECHENMACHER, C.; POLACCO, J. C.; STOLF, R.; MARCELINO, F. C.; ABDELNOOR, R. V.; HOMRICH, M. S.; DEL PONTE, E. M.; CARLINI, C. R.; CARVALHO, M. C. C. G. de; BODANESE-ZANETTINI, M. H. |
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Afiliação: |
BEATRIZ WIEBKE-STROHM, UFRGS; GIANCARLO PASQUALI, UFRGS; MÁRCIA MARGIS-PINHEIRO, UFRGS; MARTA BENCKE, UFRGS; LAURO BÜCKER NETO, UFRGS; ARLETE B. BECKER-RITT, UFRGS; ANNE H. S. MARTINELLI, UFRGS; CILIANA RECHENMACHER, UFRGS; JOSEPH C. POLACCO, University of Missouri; RENATA STOLF, CNPSo; FRANCISMAR CORREA MARCELINO, CNPSO; RICARDO VILELA ABDELNOOR, CNPSO; MILENA S. HOMRICH, UFRGS; EMERSON M. DEL PONTE, UFRGS; CELIA R. CARLINI, UFRGS; MAYRA C. C. G. DE CARVALHO, CNPSo; MARIA HELENA BODANESE-ZANETTINI, UFRGS. |
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Título: |
Ubiquitous urease affects soybean susceptibility to fungi. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Plant Molecular Biology, The Hague, v. 79, n. 1/2, p. 75-87, May 2012. |
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DOI: |
DOI 10.1007/s11103-012-9894-1 |
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Idioma: |
Inglês |
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Conteúdo: |
The soybean ubiquitous urease (encoded by GmEu4) is responsible for recycling metabolically derived urea. Additional biological roles have been demonstrated for plant ureases, notably in toxicity to other organisms. However, urease enzymatic activity is not related to its toxicity. The role of GmEu4 in soybean susceptibility to fungi was investigated in this study. A differential expression pattern of GmEu4 was observed in susceptible and resistant genotypes of soybeans over the course of a Phakopsora pachyrhizi infection, especially 24 h after infection. Twenty-nine adult, transgenic soybean plants, representing six independently transformed lines, were obtained. Although the initial aim of this study was to overexpress GmEu4, the transgenic plants exhibited GmEu4 co-suppression and decreased ureolytic activity. The growth of Rhizoctonia solani, Phomopsis sp., and Penicillium herguei in media containing a crude protein extract from either transgenic or non-transgenic leaves was evaluated. The fungal growth was higher in the protein extracts from transgenic urease-deprived plants than in extracts from nontransgenic controls. When infected by P. pachyrhizi uredospores, detached leaves of urease-deprived plants developed a significantly higher number of lesions, pustules and erupted pustules than leaves of non-transgenic plants containing normal levels of the enzyme. The results of the present work show that the soybean plants were more susceptible to fungi in the absence of urease. It was not possible to overexpress active GmEu4. For future work, overexpression of urease fungitoxic peptides could be attempted as an alternative approach. MenosThe soybean ubiquitous urease (encoded by GmEu4) is responsible for recycling metabolically derived urea. Additional biological roles have been demonstrated for plant ureases, notably in toxicity to other organisms. However, urease enzymatic activity is not related to its toxicity. The role of GmEu4 in soybean susceptibility to fungi was investigated in this study. A differential expression pattern of GmEu4 was observed in susceptible and resistant genotypes of soybeans over the course of a Phakopsora pachyrhizi infection, especially 24 h after infection. Twenty-nine adult, transgenic soybean plants, representing six independently transformed lines, were obtained. Although the initial aim of this study was to overexpress GmEu4, the transgenic plants exhibited GmEu4 co-suppression and decreased ureolytic activity. The growth of Rhizoctonia solani, Phomopsis sp., and Penicillium herguei in media containing a crude protein extract from either transgenic or non-transgenic leaves was evaluated. The fungal growth was higher in the protein extracts from transgenic urease-deprived plants than in extracts from nontransgenic controls. When infected by P. pachyrhizi uredospores, detached leaves of urease-deprived plants developed a significantly higher number of lesions, pustules and erupted pustules than leaves of non-transgenic plants containing normal levels of the enzyme. The results of the present work show that the soybean plants were more susceptible to fungi in the absence of u... Mostrar Tudo |
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Palavras-Chave: |
Penicillium herguei; Transformação genética. |
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Thesagro: |
Fungo; Phomopsis; Queima da saia; Soja. |
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Thesaurus NAL: |
Genetic transformation; Soybeans. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02843naa a2200421 a 4500
001 1917463
005 2017-01-19
008 2012 bl uuuu u00u1 u #d
024 7 $aDOI 10.1007/s11103-012-9894-1$2DOI
100 1 $aWIEBKE-STROHM, B.
245 $aUbiquitous urease affects soybean susceptibility to fungi.
260 $c2012
520 $aThe soybean ubiquitous urease (encoded by GmEu4) is responsible for recycling metabolically derived urea. Additional biological roles have been demonstrated for plant ureases, notably in toxicity to other organisms. However, urease enzymatic activity is not related to its toxicity. The role of GmEu4 in soybean susceptibility to fungi was investigated in this study. A differential expression pattern of GmEu4 was observed in susceptible and resistant genotypes of soybeans over the course of a Phakopsora pachyrhizi infection, especially 24 h after infection. Twenty-nine adult, transgenic soybean plants, representing six independently transformed lines, were obtained. Although the initial aim of this study was to overexpress GmEu4, the transgenic plants exhibited GmEu4 co-suppression and decreased ureolytic activity. The growth of Rhizoctonia solani, Phomopsis sp., and Penicillium herguei in media containing a crude protein extract from either transgenic or non-transgenic leaves was evaluated. The fungal growth was higher in the protein extracts from transgenic urease-deprived plants than in extracts from nontransgenic controls. When infected by P. pachyrhizi uredospores, detached leaves of urease-deprived plants developed a significantly higher number of lesions, pustules and erupted pustules than leaves of non-transgenic plants containing normal levels of the enzyme. The results of the present work show that the soybean plants were more susceptible to fungi in the absence of urease. It was not possible to overexpress active GmEu4. For future work, overexpression of urease fungitoxic peptides could be attempted as an alternative approach.
650 $aGenetic transformation
650 $aSoybeans
650 $aFungo
650 $aPhomopsis
650 $aQueima da saia
650 $aSoja
653 $aPenicillium herguei
653 $aTransformação genética
700 1 $aPASQUALI, G.
700 1 $aMARGIS-PINHEIRO, M.
700 1 $aBENCKE, M.
700 1 $aBÜCKER-NETO, L.
700 1 $aBECKER-RITT, A. B.
700 1 $aMARTINELLI, A. H. S.
700 1 $aRECHENMACHER, C.
700 1 $aPOLACCO, J. C.
700 1 $aSTOLF, R.
700 1 $aMARCELINO, F. C.
700 1 $aABDELNOOR, R. V.
700 1 $aHOMRICH, M. S.
700 1 $aDEL PONTE, E. M.
700 1 $aCARLINI, C. R.
700 1 $aCARVALHO, M. C. C. G. de
700 1 $aBODANESE-ZANETTINI, M. H.
773 $tPlant Molecular Biology, The Hague$gv. 79, n. 1/2, p. 75-87, May 2012.
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Embrapa Soja (CNPSO) |
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